@article { author = {Aghlmand, Saeideh and Nasiri, Esmail and Mohammad, Rahmani}, title = {Negative effect of high-calorie high-salt diet on spleen oxidant/antioxidants and structure: preventive effects of sprint interval training}, journal = {Journal of Basic and Clinical Pathophysiology}, volume = {10}, number = {2}, pages = {9-17}, year = {2022}, publisher = {Shahed University}, issn = {2322-1895}, eissn = {2345-4334}, doi = {10.22070/jbcp.2022.17049.1163}, abstract = {Objective: We investigated the effect of a high-calorie high-salt diet and sprint interval training on oxidant/antioxidant status and structural changes in the spleen of male Wistar rats.Materials and Methods: Eighteen male Wistar rats were randomized into three groups: normal diet (ND), high-calorie high-salt diet (HCSD), and HCSD + sprint interval training (HCSD+SIT). Rats in HCSD and HCSD+SIT groups were under a high-calorie high-salt diet. The SIT (4-9 reps of 10 s duration sprints) performed 3 sessions/week for 8 weeks. Forty-eight hours after the last training session, spleen was removed and used for the assessment of oxidant/antioxidant status and histomorphometric parameters.Results: Results revealed that compared to the ND group, superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) level, and total antioxidant capacity (TAC) were significantly lower (P<0.001) and malondialdehyde (MDA) level was significantly higher in the spleen tissue of the HCSD group (P<0.001) and there was no significant difference between the HCSD+SIT and ND groups in none of the assessed variables (P>0.05). Moreover, there was a significant increase in the red to white pulp ratio and a significant decrease in the number of lymph cells and splenic trabecular volume in the HCSD group (P<0.001). Nonetheless, in none of the assessed morphometric parameters, a significant difference was observed between the HCSD+SIT and ND groups (P>0.05).Conclusion: It seems that SIT training may avert HCSD-induced unfavourable changes in the spleen oxidant/antioxidant status and counteract the deleterious effects of the HCSD on the spleen structure.}, keywords = {High-fat diet,High-sugar diet,High-salt diet,High-Intensity interval training,Spleen}, url = {https://jbcp.shahed.ac.ir/article_4033.html}, eprint = {https://jbcp.shahed.ac.ir/article_4033_c656fc339a087531182bb17a50ac3fa6.pdf} }