Shahed University Journal of Basic and Clinical Pathophysiology 2322-1895 5 1 2017 03 01 The effect of methadone and haloperidol combination on anxiety induced by morphine withdrawal in male mice 19 26 437 10.22070/jbcp.2017.2267.1074 EN Ashkan Sanaie Rad Shahed University Elham Zahedi shahed University Fariba Ansari Shahed University Mohsen Khalili department of Physiology,school of medicine,shahed university,Abdollah Zadeh street, Keshavarz Blvd,Tehran, Iran Journal Article 2017 01 02 <strong>Background and Objective:</strong> Regarding inefficiency of common drugs used for alleviation of anxiety due to narcotics withdrawal, the present study was evaluated methadone and haloperidol co-drugs therapy on anxiety due to morphine withdrawal.<br /> <strong>Materials and Methods:</strong> Ninety eight NMRI male mice were divided into acute and chronic experimental groups. Then, each group was divided into 7 subgroups: saline, morphine (control), methadone, haloperidol, methadone+haloperidol, methadone+haloperidol with 2/1 and 1/2 ratio, respectively. Mice were addicted chronically (over 8 days) by receiving escalating doses of morphine and acute (morphine was applied only on 8^th day) procedures. Anxiety was induced by naloxone application in addicted mice. Elevated plus-maze and open field tests were used for evaluation of anxiety<strong>. </strong><br /> <strong>Results:</strong> Obtained data showed that in both chronic and acute groups, treatment with co-drugs methadone and haloperidol could markedly alleviate anxiety signs produced by interruption of morphine consumption.<br /> <strong>Conclusion:</strong> We found out that the anxiety as a major sign of morphine withdrawal sign could be diminished by methadone+haloperidol therapy versus drugs alone. https://jbcp.shahed.ac.ir/article_437_bd7cae62b030e797d82fce4aee560d82.pdf