Shahed University Journal of Basic and Clinical Pathophysiology 2322-1895 12 2 2024 10 30 Determination of most frequent major histocompatibility complex alleles in Iranian population by immunoinformatic 38 42 4611 10.22070/jbcp.2024.19457.1182 EN Fatemeh Hajighasemi Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran Atefeh Shirkavand Department of Genetics, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran Journal Article 2024 08 10 <strong>Background and Objective:</strong> Major histocompatibility complex (MHC) is the most polymorphic genes in human genome encoding human leukocyte antigens (HLA) class I and class II. Determination of HLA alleles could help in preparing more efficient vaccines as well as successful organ transplantation in different population of the world. Immunoinformatic uses biologic computational information for modelling and prediction of immune system function in healthiness and diseases. In this study we determined the most frequent MHC alleles in Iranian population by immunoinformatic.<br /><strong>Materials and Methods:</strong> Utmost frequent human leukocyte antigens (HLA) alleles in Iranian population were determined using allele frequency net database (AFND, www.allelefrequencies.net).<br /><strong>Results:</strong> A total of ten alleles related to HLA Class I ( five  alleles related to HLA –A [A02 (16%), A03 (12.5%), A11(10%), A24 (13.6%), A26 (10%)], three alleles related to HLA –B [(B35 (13%), B510 (12%), B53(13)%)] and two  alleles related to HLA –C [C07 (17.5%), C12 (16%)] and a total of four alleles related to HLA Class II (two  alleles related to HLA- DQB1 [DQB1 03 (30.5%), DQB1 05 (22 %)] and two  alleles related to HLA- DRB1 [DRB1 04 (17.1%), DRB1 15 (16.1 %)], were utmost frequent alleles in the Iranian population.  <br /><strong>Conclusion:</strong> In this study most frequent HLA alleles in Iranian population were found by immunoinformatic. These alleles could be very valuable for development of more effective vaccines as well as successful bone marrow transplantation in Iran. Besides these data could have potential implication for finding the best matched un-related donors in Iranian populations. https://jbcp.shahed.ac.ir/article_4611_38cd2c728bc22944561a34b8b7995a07.pdf