Shahed University Journal of Basic and Clinical Pathophysiology 2322-1895 13 1 2025 02 25 Antiepileptic effect of nobiletin in PTZ-induced catamenial seizures in the rat 44 49 4720 10.22070/jbcp.2025.20006.1193 EN Mohadeseh Sharifian Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran Shahin Hassanpour Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran 0000-0002-4417-1819 Journal Article 2025 01 09 <strong>Background and Objective:</strong> Catamenial epilepsy is a seizure pattern associated with the menstrual cycle that happens in specific women with epilepsy while menstruating. Nobiletin is a type of polymethoxy flavonoid and with many beneficial physiological effects. This study aimed to determine antiepileptic effect of nobiletin in PTZ-induced catamenial seizures in rats.<br /><strong>Materials and Methods:</strong> Thirty female Wistar rats, separated into 5 experimental groups based on their estrus cycle stage (proestrus, estrus, metestrus, diestrus). In the control group, rats received saline, while in experiment 2, the animals were given VPA (75 mg/kg). During experiments 3-5, animals received nobiletin at doses 12.5, 25, and 50 mg/kg, respectively. Then, the animals received an 80 mg per kilogram intraperitoneal injection of PTZ (80 mg/kg). Following the start of the seizure, the animals' actions were observed for a period of 30 minutes to evaluate the onset of ITMS and ITTS. Experiments were conducted between 9 a.m. and 12 p.m. to minimize the influence of circadian rhythm on vulnerability to seizures.<br /><strong>Results:</strong> Based on findings, nobiletin (20 and 30 mg/kg) increased the initiation time myoclonic seizures (ITMS) and initiation time of tonic-clonic seizures (ITTS) and decreased seizure duration (SD) after PTZ administration in rats during different stages of the estrous cycle (p<0.05). The effects of nobiletin were more pronounced during metestrus and diestrus in comparison to proestrus and estrus (p<0.05).<br /><strong>Conclusion:</strong> Findings suggested that nobiletin has antiepileptic effect in PTZ-induced catamenial seizure in rat. https://jbcp.shahed.ac.ir/article_4720_4ff4cc37d2a4a18ad658cd74f297b6d3.pdf