Journal of Basic and Clinical Pathophysiology

Journal of Basic and Clinical Pathophysiology

Antimicrobial resistance and molecular typing of Pseudomonas aeruginosa and Acinetobacter baumanii isolated from a burn hospital in Tehran, Iran

Document Type : Research Paper

Authors
Mohammad Mehdi Attarpour Yazdi 1
Sareh sadat Hosseini 2
1 Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, IranTehran, Iran
2 Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
10.22070/jbcp.2019.4544.1118
Abstract
Background and Objective: Acinetobacter baumanii and pseudomonas aeruginosa are common opportunistic gram-negative bacteria related to hospital-acquired infections. Multidrug resistant microorganisms have emerged as the causes of nosocomial infections worldwide. In this study, we evaluated the existence of blaTEM, blaSHV and blaCTX-M genes among Pseudomonas aeruginosa and Acinetobacter baumannii strains isolated from hospitalized patients in one burn hospital in Tehran, Iran.
Materials and Methods: From June 2015 to May 2016, 82 isolates were collected from burn patients hospitalized in one burn hospital in Tehran, Iran. A total of 82 isolates of gram-negative, non-fermentative bacilli including pseudomonas aeruginosa (58 isolates) and Acinetobacter baumanii (24 isolates) were tested for susceptibility to selective antibiotics by disk diffusion recommended in CLSI guidelines. All the resistant isolates were subjected to PCR assay for blaTEM, blaSHV, and blaCTX-M genes that encode ESBL.
Results: Resistance to gentamicin was 83%, but resistance to cephalosporins was higher than gentamicin. Out of the 47 pseudomonas aeruginosa resistance isolates, 24 and 9 isolates were CTX-M and TEM procedure, respectively. Among 8 Acinetobacter baumanii isolates that were resistant to all antibiotics, 7 and 1 isolates were CTX-M and TEM producer, respectively.In addition, bla SHV gene was not detected in any of the isolates.
Conclusion: High level of resistance to most antibiotics tested and high prevalence of bla CTX-M gene in this study, indicating the careful detection of antimicrobial resistant strains is needed in order to prevent further resistance to antimicrobial agents in Iran.
Keywords
Pseudomonas aeruginosa
Acinetobacter baumanii
bla CTXM
blaTEM
bla SHV
  1. Sharma R, Sharma CL, Kapoor B. Antibacterial resistance:current problems and possible solutions. Indian Journal of Medical Sciences 2005; 59: 120 - 129.
  2. Poirel L, Menuteau O, Agoli N, Cattoen C, Nord-mann P. Outbreak of Extended-Spectrum β-LactamaseVEB-1-Producing Isolates of Acinetobacter baumanniiina French Hospital. Journal of Clinical Microbiology 2003; 41: 3542-3547.
  3. Xu Z, Li L, Shirtliff ME, Alam MJ, Yamasaki S, Shi L . Occurrence and characteristics of class 1 and 2 integrons in Pseudomonas aeruginosa isolates from patients in southern China. Journal of Clinical Microbiology 2009; 47: 230 - 234.
  4. Li J, Rayner CR, Nation RL, Owen RJ, Spelman D, Tan KE, et al. Heteroresistance to colistin in multidrug-resistant Acinetobacterbaumannii. Journal of Antimicrobial Agents and Chemoterapy 2006; 50: 2946 – 2950.
  5. Qarah S, Cunha AB, Dua P, et al. Pseudomonas aeruginosa infections. 2008. [Cited 2011 Dec 15]. Available from: URL: http://www. emedicine.com /med/topic1943.html.
  6. Lister P, Wolter D, Hanson N. Antibacterial-Resistant Pseudomonas aeruginosa: Clinical Impact and ComplexRegulation of Chromosomally Encoded Resistance Mechanism. Clinical Microbiology Reviews 2009; 22(4): 582- 610.
  7. Paterson DL, Bonomo RA. Extended - Spectrum ß -Lactamases: A Clinical Update. Clinical Microbiology Reviews. 2005; 18(4):657- 686.
  8. Perez F, Hujer AM, Hujer KM, Decker BK, Rather PN, Bonomo RA. Global challenge of multidrug resistant Acinetobacter baumanii. Journal of Antimicrobial Agents and Chemoterapy. 2007; 51(10): 3471- 3484.
  9. Dhillon RH, Clark J. ESBLs: a clear and present danger?. Critical Care Research and Practice Journal. 2011; 2012.
  10. N., M. J. Ellington, J. M. Coelho, J. F. Turton, M. E. Ward, S. Brown, S. G. B. Amyes, and D. M. Livermore.. Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp. journal of Antimicrobial Agents and Chemoterapy 2006; 27:351-353.
  11. Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing. Twentieth informational supplement .M100-S20. Wayne, PA 19087, USA; 2010.
  12. Yan JJ, Tsai SH, Chuang CL, Wu JJ. OXA-type betalactamases among extended-spectrum cephalosporin resistant Pseudomonas aeru­ginosa isolates in a university hospital in southern Taiwan. Journal of Microbiology, Immunology and Infection 2006; 39: 130- 134.
  13. Jemima SA, Verghese S. Multiplex PCR for bla CTX-M & blaSHV in the extended-spectrum beta lactamase (ESBL) producingGram-negative isolates. Indian Journal of Medical Research 2008; 128:313–317.
  14. Jiang X, Zhang Z, Li M, Zhou D, Ruan F, Lu Y: Detection of extended-spectrum beta-lactamases in clinical isolates of Pseudomonas aeruginosa. Journal of Antimicrobial Agents and Chemoterapy 2006; 50: 2990–2995.
  15. Weldhagen GF, Poirel L, Nordmann P. Ambler class A extended-spectrum beta-lactamases in Pseudomonas aeruginosa: novel developments and clinical impact. Journal of Antimicrobial Agents and Chemoterapy 2003; 47(8):2385-2392.
  16. Peleg A. Y, Seifert H, Paterson D. L. Acinetobacter baumanii: emergence of a successful pathogen. Clinical Microbiology Reviews 2008; 21:538 - 582.
  17. Perez F, Hujer AM, Hujer K. M, Decker B. K., Rather P. N, Bonomo R. A. Global challenge of multidrug-resistant Acinetobacter baumannii. Journal of Antimicrobial Agents and Chemoterapy 2007; 51:3471- 3484.
  18. Polloto M ,Casella T,Oliveria M,Rubio F,Nogureria M ,Almedia M,et al . Detection of P. aeruginosa harboring blaCTX-M-2, blaGES-1 and blaGES-5, blaIMP-1 and blaSPM1 causinginfections in Brazilian tertiarycare hospital. Journal of BMC Infectious Diseases 2012; 12:176-179.
  19. Picao R, Poirel L, Gales A, Nordmann P. Diversity of Beta-Lactamases Produced by Ceftazidime-Resistant Pseudomonas aeruginosa Isolates Causing Bloodstream Infections in Brazil. Journal of Antimicrobial Agents and Chemoterapy 2009; 53(9): 3908 - 3913.
  20. Shahcheraghi F, Nikbin VS, Feizabadi MM. Prevalence of ESBLs genes among multidrug-resistant isolates of Pseudomonas aeruginosa isolated from patients in Tehran. Journal of Microbial Drug Resistance 2009; 15(1): 37- 39.
  21. Celenza G, Pellegrini C, Caccamo M , Segatore B, Amicosante G , Perilli M .Spread of blaCTX-M-type and blaPER-2 b-lactamasegenes in clinical isolates from Bolivian hospitals. Journal of Antimicrobial and Chemotherapy 2006; 57: 975 - 978.
  22. Hujer K , Hujer A , Hulten E, Bajaksouzian S , Adams J, Donskey C, et al. Analysis of Antibiotic Resistance Genes in Multidrug Resistant Acinetobacter sp. Isolates from Military and Civilian Patients Treated at the Walter Reed Army Medical Center. Journal of Antimicrobial and Chemotherapy 2006; 50(12): 4114 - 4123.
  23. JIN Hui, XU Xiao-min, MI Zu-huang, MOU Yi. LIU Pei, Drug resistant gene base genotyping for Acinetobacter baumanii in tracing epidemiological events and for clinical treatment within nosocomial settings. Journal of Chinese Medicine 2009; 122(3): 301-306.
Journal of Basic and Clinical Pathophysiology
Volume 7, Issue 2
December 2019
Pages 40-43