Journal of Basic and Clinical Pathophysiology

Journal of Basic and Clinical Pathophysiology

The effect of nobiletin on performance of rats in forced swimming and elevated plus maze tests in intranigral lipopolysaccharide rat model of Parkinson's disease

Document Type : Research Paper

Authors
Maryam Khorasani 1
Zahra Kiasalari 2
Reihane Ghasemi 1
Marzieh Fakour 1
Sedigheh Keshtkar 1
Mehrdad Roghani 2
1 Department of Physiology, School of Medicine, Shahed University, Tehran, Iran
2 Neurophysiology Research Center, Shahed University, Tehran, Iran
10.22070/jbcp.2020.4355.1115
Abstract
Background and Objective: Anti-inflammatory property of nobiletin (NOB) is proven and neuroinflammation is involved in triggering and progression of neurodegenerative disorder such as Parkinson's disease (PD). PD is a neurodegenerative disorder characterized by motor and non-motor features including psychiatric symptoms such as depression and anxiety. The purpose of this study to investigate whether oral nobiletin administration at a dose of 10 mg/kg has the ability to alleviate non-motor behavioural changes including depression and anxiety-like behaviors in LPS-induced model of PD in rat.
Materials and Methods: For this purpose, 32 male Wistar rats (195-245 g) were divided into four groups (n=8) as follows: Sham-operated group, nobiletin-treated sham-operated group (sham+NOB), lesion group (LPS) and nobiletin-treated lesion group (LPS+NOB). LPS (5 μg/kg) rat was unilaterally injected into the SN of rat brains through standard stereotaxis, according to the atlas of Paxinos and Watson (to generate a neuroinflammatory model of PD), with or without NOB (10 mg/kg administrated daily for 1 week after surgery, via gavage). Behavioral assessment was carried out one week after surgery by assessment of performance in forced swimming and elevated plus maze tests.
Results: NOB-treated LPS group showed significant decrease in immobility time and insignificant increase in the percentage of open arm spending time as compared with LPS group which demonstrate the anti-depressant like effect of NOB in inflammatory model of PD in rats.
Conclusion: Taken together, this study demonstrated that nobiletin as an anxiolytic and anti-depressive agent in the LPS-induced rat model of Parkinson’s disease.
Keywords
Parkinson’s disease
Nobiletin
Neuroinflammation
Behavioral changes
Lipopolysaccharide
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