Journal of Basic and Clinical Pathophysiology

Journal of Basic and Clinical Pathophysiology

Dose-dependent effect of Hypericum perforatum extract on motor imbalance following intrastriatal injection of 6-hydroxydopamine in the rat

Authors
Siamak Afshin-Majd 1
Mohsen Khalili 1
Zahra Kiasalari 1
Batool Rahmati* 1
Manijeh Karami 1
Gholamhossein Ghaedi 1
Tourandokht Baluchnejadmojarad 2
Mehrdad Roghani 1
Fariba Ansari 3
1 Neurophysiology Research Center, Shahed University, Tehran, Iran
2 Department of Physiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
3 Department of Physiology, School of Medicine, Shahed University, Tehran, Iran
10.22070/jbcp.2016.281
Abstract
Background and Objective: Parkinson's disease (PD) is a neurodegenerative disease with selective damage of mesencephalic dopaminergic neurons. Due to the protective, anti-inflammatory, and antioxidant effect of Hypericum perforatum (HP), this study was undertaken to assess dose-dependent effect of HP hydroalcoholic extract on motor imbalance following intrastriatal injection of 6-hydroxydopamine in the rat.
Materials and Methods: In this experimental research, male Wistar rats (n=35) were equally divided into sham, 6-hydroxydopamine (OHDA)-lesioned, and HP extract-treated lesion groups. Model of PD was induced by microinjecting 12.5 microgram of 6-OHDA dissolved in saline-ascorbate solution into the left striatum. Treated lesion groups received HP extract at doses of 50, 100, and 200 mg/kg/day p.o. started one week before the surgery for 1 week post-surgery. After 1 week, ipsilateral and contralateral rotations induced by apomorphine were counted and net scores were obtained.
Results: In the 6-OHDA-lesioned group, the dopaminergic agonist apomorphine induced contralateral rotational behavior (PConclusion: Oral administration of HP extract at doses of 100 and 200 mg/kg could reduce motor imbalance and attenuate forced biased rotational behavior in 6-OHDA-induced model of PD.
Keywords
Hypericum perforatum 6-hydroxydopamine Rotational behavior Motor imbalance Dose-dependent
Journal of Basic and Clinical Pathophysiology
Volume 4, Issue 1 - Serial Number 1
November 2015
Pages 23-26