Journal of Basic and Clinical Pathophysiology

Journal of Basic and Clinical Pathophysiology

The effect of l-theanine administration on testicular ischemia/reperfusion injury in the rat

Document Type : Research Paper

Authors
Mahsa Suleghani 1
Ahmad Asghari 1
Shahin Hassanpour 2
Alireza Jahandideh 1
1 Department of Clinical Science, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Basic Science, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
10.22070/jbcp.2022.16049.1155
Abstract
Background and Objective: Ischemia-reperfusion (IR) injury is one of the main pathophysiologic conditions of the testis. Despite free radical scavenging property of the tannins, limit information exists for their antioxidant activity. The aim of this study was to determine the effect of L-theanine administration on testicular I/R injury in the rat.
Materials and Methods: Thirty male Wistar rats were randomly divided into 5 experimental groups (n=6/group). In I/R group, rats subjected to 4 h I/20 h R period and i.p. injected with saline 1 hour before the R. In groups 3-5, rats subjected to 4 h I/20 h R period and i.p. injected with L-theanine (100, 200 or 400 mg/kg) 1 hour before the R. At the end of the study, left testis was removed for histological analysis and antioxidant measurement.
Results: According to the results, I/R leads to degenerated seminiferous tubules and loss of spermatogenesis. Administration of the L-theanine (200 and 400 mg/kg) followed by I/R was associated with many normal seminiferous tubules. Tissue malondialdehyde (MDA) levels significantly increased in I/R rats (P<0.05) while L-theanine decreased I/R-induced MDA (P<0.05). Experimental I/R significantly decreased superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity (P<0.05). Administration of the L-theanine significantly increased tissue activity of SOD and GPx in I/R rat (P<0.05).
Conclusion: These findings suggested that treatment with L-theanine has a beneficial effect against I/R
Keywords
Ischemia
L-theanine
Reperfusion
Testicular
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Journal of Basic and Clinical Pathophysiology
Volume 10, Issue 1
June 2022
Pages 17-24