The effect of l-theanine administration on testicular ischemia/reperfusion injury in the rat

Document Type : Research Paper

Authors

1 Department of Clinical Science, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Basic Science, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

Background and Objective: Ischemia-reperfusion (IR) injury is one of the main pathophysiologic conditions of the testis. Despite free radical scavenging property of the tannins, limit information exists for their antioxidant activity. The aim of this study was to determine the effect of L-theanine administration on testicular I/R injury in the rat.
Materials and Methods: Thirty male Wistar rats were randomly divided into 5 experimental groups (n=6/group). In I/R group, rats subjected to 4 h I/20 h R period and i.p. injected with saline 1 hour before the R. In groups 3-5, rats subjected to 4 h I/20 h R period and i.p. injected with L-theanine (100, 200 or 400 mg/kg) 1 hour before the R. At the end of the study, left testis was removed for histological analysis and antioxidant measurement.
Results: According to the results, I/R leads to degenerated seminiferous tubules and loss of spermatogenesis. Administration of the L-theanine (200 and 400 mg/kg) followed by I/R was associated with many normal seminiferous tubules. Tissue malondialdehyde (MDA) levels significantly increased in I/R rats (P<0.05) while L-theanine decreased I/R-induced MDA (P<0.05). Experimental I/R significantly decreased superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity (P<0.05). Administration of the L-theanine significantly increased tissue activity of SOD and GPx in I/R rat (P<0.05).
Conclusion: These findings suggested that treatment with L-theanine has a beneficial effect against I/R

Keywords

References

  1. Tanriverdi HI, Şenel U, Gevrek F, Akbaş A. Protective effect of famotidine on ischemia–reperfusion injury following testicular torsion in rats. Journal of Pediatric Urology 2021;17(2):167-e1.
  2. Chouchani ET, Pell VR, James AM, Work LM, Saeb-Parsy K, Frezza C, et al. A unifying mechanism for mitochondrial superoxide production during ischemia-reperfusion injury. Cell Metabolism 2016 ;23(2):254-63.
  3. Lee JW, Hwang ET, Han JS. Protective Effects of Topical Application of Nitrite on Testicular Ischemia-Reperfusion Injury in Rats. Oxidative Medicine and Cellular Longevity 2021.
  4. Hidese S, Ogawa S, Ota M, Ishida I, Yasukawa Z, Ozeki M, Kunugi H. Effects of L-theanine administration on stress-related symptoms and cognitive functions in healthy adults: a randomized controlled trial. Nutrients 2019;11(10):2362.
  5. Saeed M, Khan MS, Kamboh AA, Alagawany M, Khafaga AF, Noreldin AE, et al. L-theanine: an astounding sui generis amino acid in poultry nutrition. Poultry Science 2020.
  6. Fu X, Liao Y, Cheng S, Xu X, Grierson D, Yang Z. Nonaqueous fractionation and overexpression of fluorescent‐tagged enzymes reveals the subcellular sites of L‐theanine biosynthesis in tea. Plant Biotechnology Journal 2021;19(1):98.
  7. Kiasalari Z, Rahmani T, Mahmoudi N, Baluchnejadmojarad T, Roghani M. Diosgenin ameliorates development of neuropathic pain in diabetic rats: involvement of oxidative stress and inflammation. Biomedicine & Pharmacotherapy 2017;86:654-61.
  8. Li G, Ye Y, Kang J, Yao X, Zhang Y, Jiang W, Gao M, Dai Y, Xin Y, Wang Q, Yin Z. L-Theanine prevents alcoholic liver injury through enhancing the antioxidant capability of hepatocytes. Food and Chemical Toxicology 2012;50(2):363-72.
  9. Thangarajan S, Deivasigamani A, Natarajan SS, Krishnan P, Mohanan SK. Neuroprotective activity of L-theanine on 3-nitropropionic acid-induced neurotoxicity in rat striatum. International Journal of Neuroscience 2014;124(9):673-84.
  10. Deb S, Dutta A, Phukan BC, Manivasagam T, Thenmozhi AJ, Bhattacharya P, Paul R, Borah A. Neuroprotective attributes of L-theanine, a bioactive amino acid of tea, and its potential role in Parkinson's disease therapeutics. Neurochemistry International 2019;129:104478.
  11. Ye H, Liu Z, Zhou L, Cai Q. Dynamic Observation of the Effect of L-Theanine on Cerebral Ischemia-Reperfusion Injury Using Magnetic Resonance Imaging under Mathematical Model Analysis. Journal of Healthcare Engineering 2021.
  12. Zukhurova M, Prosvirnina M, Daineko A, Simanenkova A, Petrishchev N, Sonin D, et al. L‐theanine administration results in neuroprotection and prevents glutamate receptor agonist‐mediated injury in the rat model of cerebral ischemia‐ Phytotherapy Research 2013;27(9):1282-7.
  13. Turkili B, Kurcer Z, Dengiz GO, Kandemir NO, Mungan G, Ozacmak VH, et al. Role of angiotensin and endothelin in testicular ischemia reperfusion injury. International Journal of Urology 2012;19(3):257-63.
  14. Koksal M, Oğuz E, Baba F, Eren MA, Ciftci H, Demir ME, et al. Effects of melatonin on testis histology, oxidative stress and spermatogenesis after experimental testis ischemia-reperfusion in rats. European Review for Medical and Pharmacological Sciences 2012;16(5):582-8.
  15. Sahin Z, Bayram Z, Celik-Ozenci C, Akkoyunlu G, Seval Y, Erdogru T, et al. Effect of experimental varicocele on the expressions of Notch 1, 2, and 3 in rat testes: an immunohistochemical study. Fertility and Sterility 2005;83(1):86-94.
  16. Minutoli L, Antonuccio P, Romeo C, Nicotina PA, Bitto A, Arena S, et al. Evidence for a role of mitogen-activated protein kinase 3/mitogen-activated protein kinase in the development of testicular ischemia-reperfusion injury. Biology of Reproduction 2005 Oct 1;73(4):730-6.
  17. Johnsen SG. Testicular biopsy score count–a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Hormone Research in Paediatrics 1970;1(1):2-5.
  18. Tuglu D, Yuvanc E, Yılmaz E, Gencay IY, Atasoy P, Kisa U, et al. The antioxidant effect of dexmedetomidine on testicular ischemia-reperfusion injury. Acta Cirurgica Brasileira 2015;30:414-21.
  19. Abdallah HM. New trends in pharmacological treatment of acute kidney injury. Asian Pacific Journal of Tropical Biomedicine 2021;11(7):285.
  20. Zadeh JK, Garcia-Bardon A, Hartmann EK, Pfeiffer N, Omran W, Ludwig M, Patzak A, Xia N, Li H, Gericke A. Short-time ocular ischemia induces vascular endothelial dysfunction and ganglion cell loss in the pig retina. International Journal of Molecular Sciences 2019;20(19):4685.
  21. Shamsi-Gamchi N, Razi M, Behfar M. Testicular torsion and reperfusion: evidences for biochemical and molecular alterations. Cell Stress and Chaperones 2018;23(3):429-39.
  22. Ďuračková Z. Systems biology of free radicals and antioxidants. In Free radicals and antioxidants for non-experts 2014; 3-38. Berlin: Springer.
  23. Samad N, Jabeen S, Imran I, Zulfiqar I, Bilal K. Protective effect of gallic acid against arsenic-induced anxiety−/depression-like behaviors and memory impairment in male rats. Metabolic Brain Disease 2019;34(4):1091-102.
  24. Das SK, Karmakar SN. Effect of green tea (camellia sinensis l.) leaf extract on reproductive system of adult male albino rats. International Journal of Physiology, Pathophysiology and Pharmacology 2015;7(4):178.
  25. Chandra AK, Choudhury SR, De N, Sarkar M. Effect of green tea (Camellia sinensis L.) extract on morphological and functional changes in adult male gonads of albino rats. Indian Journal of Experimental Biology 2011;49:689–97.
  26. Opuwari C, Monsees T. Green tea consumption increases sperm concentration and viability in male rats and is safe for reproductive, liver and kidney health. Scientific Reports 2020;10(1):1-4.
Journal of Basic and Clinical Pathophysiology
Volume 10, Issue 1
June 2022
Pages 17-24

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