Effectiveness of Fe2O3 nanoparticles more than magnetic field against the destructive effect of colchicine on Paramecium caudatum

Document Type : Research Paper

Authors

1 Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran Iran

2 Department of Physics, Faculty of Basic Sciences, Shahed University, Tehran, Iran

Abstract

Background and Objective: Colchicine depolymerizes microtubules and reduces their diamagnetic capacity. We investigated the effect of a static magnetic field (SMF) and the Fe2O3 NPs on the Paramecium caudatum exposed to colchicine.
Materials and Methods: The samples were collected from temporary water sources and after identifying the species, were cultured in the laboratory. They were also sub-cultured at regular intervals (7-10 days) for purification. From pure culture, several samples were placed in the laboratory or under SMF with an intensity of 61 mT for 3 days. To evaluate the effect of materials in comparison with distilled water (control), a sample (0.1 ml) of each medium was placed on a slide and exposed to 1 μl of distilled water or volume-concentrations of colchicine (0.05 to 25 μg/μl) or Fe2O3 NPs (0.05 to 3 μg/μl). The effect of different concentrations of Fe2O3 NPs in accompany with the field was also investigated. The movement of the animal was examined for 30 sec under a constant view and the sample was fixed for staining and study at the cellular level. Data were analyzed using analysis of variance.
Results: Colchicine at high concentrations (15 and 25 μg/μl) significantly reduced the motility of Paramecium caudatum. Magnetic field, but not NPs, alone reduced cell motility. Co-exposure to particles completely improved cell motility due to exposure to colchicine, however, in animals initially housed in the magnetic field, the healing effect of the particles was impaired.
Conclusion: The protective effects of NPs may depend on the diamagnetic capacity of the microtubule.

Keywords

References

  1. Fennell B, Naughton JA, Barlow J, Brennan GP. Microtubules as antiparasitic drug targets. Expert Opinion on Drug Discovery 2008;3(5):501-18.
  2. Trogden KP, Zhu X, Lee JS, Wright CVE, Gu G, Kaverina I. Regulation of glucose-dependent golgi-derived microtubules by cAMP/EPAC2 promotes secretory vesicle biogenesis in pancreatic b cells. Current Biology 2019;29:2339-50.
  3. Prosser, SL, Pelletier L. Mitotic spindle assembly in animal cells: a fine balancing act. Nature Reviews Molecular Cell Biology 2017;18:187-201.
  4. Qiang L, Sun X, Austin TO, Liu M, Yu W, Baas WP. Tau does not stabilize axonal microtubules but rather enables them to have long labile domains. Current Biology 2018;28:1-9.
  5. Lu Y, Chen J, Xiao M, Li W, Miller DD. An overview of tubulin inhibitors that interact with the colchicine binding site. Pharmaceutical Research 2012;29(11):2943-71.
  6. Tenuta M, Tarsitano MG, Mazzotta P, Lucchini L, Sesti F, Fattorini G, et al. Therapeutic use of pulsed electromagnetic field therapy reduces prostate volume and lower urinary tract symptoms in benign prostatic hyperplasia. Andrology 2020; 8(5):1076-1085. DOI: 10.1111/andr.12775.
  7. Sáez V, Mason TJ. Sonoelectrochemical synthesis of nanoparticles. Molecules 2009;14:4284-99. doi:10.3390/molecules14104284.
  8. Kumar Yadav V , Ali D, Heena Khan S , Gnanamoorthy G , Choudhary N , Kumar Yadav K, et al. Synthesis and characterization of amorphous iron oxide nanoparticles by the sonochemical method and their application for the remediation of heavy metals from wastewater. Nanomaterials 2020;10;1551. doi:10.3390/nano10081551.
  9. Leung YY, Hui LLY, Kraus VB. Colchicine--Update on mechanisms of action and therapeutic uses. Semin Arthritis Rheum 2015;45(3):341-50.
  10. Bhattacharyya B, Panda D, Gupta S, Banerjee M. Anti-mitotic activity of colchicine and the structural basis for its interaction with tubulin. Medicinal Research Reviews 2008;28( 1):155-83.
  11. Gray R, Gray A, Fite JL, Jordan R, Stark S, Naylor K. Simple microscopy assay to teach the processes of phagocytosis and exocytosis. CBE: Life Sciences Education 2012;11(2):180-86.
  12. Fennell BJ, Naughton JA, Barlow J, Brennan G, Fairweather I, Hoey E, McFerran N, Trudgett A, Bell A. Microtubules as antiparasitic drug targets. Expert Opinion on Drug Discovery 2008;3(5):501–18.
  13. Guevorkian K, Valles JM. Aligning Paramecium caudatum with static magnetic fields. Biophysical Journal 2006;90(8):3004–11.
  14. Guevorkian K, VallesJr JM. Aligning Paramecium caudatum with static magnetic fields. Biophysical Journal 2006;90(8):3004-11.
  15. Wang YXJ, Hussain SM, Krestin GP. Superparamagnetic iron oxide contrast agents: Physicochemical characteristics and applications in MR imaging. European Radiology 2001;11(11):2319–31.
  16. Schwaminger SP, Fraga-García P, Eigenfeld M, Becker TM, Berensmeier S. Magnetic separation in bioprocessing beyond the analytical scale: From biotechnology to the food industry. Frontiers in Bioengineering and Biotechnology 2019 Doi.org/10.3389/fbioe.2019.00233.
Journal of Basic and Clinical Pathophysiology
Volume 10, Issue 1
June 2022
Pages 30-37

Files

Share

How to cite

Statistics