S-allyl cysteine, a bioactive ingredient of Allium sativum, alleviates carbon tetrachloride-induced acute renal dysfunction in the mouse

Document Type : Research Paper


1 Department of Physiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

2 Department of Physiology, School of Medicine, Shahed University, Tehran, Iran

3 Neurophysiology Research Center, Shahed University, Tehran, Iran


Background and Objective: Kidney diseases are endangering conditions to public health. Carbon tetrachloride (CCL4)-induced model of acute kidney injury (AKI) is a reliable model for studying renal damage under different conditions. S-allyl cysteine (SAC) is a natural organosulfur compound in aged garlic extract with multiple protective effects. In this study, possible preventive effect of SAC in CCl4 model of AKI was investigated.
Materials and Methods: For induction of AKI, CCl4 (10 ml/kg body weight; 0.175% in olive oil) was intraperitoneally injected and SAC was given orally at doses of 25 or 100 mg/kg. Functional markers of kidney were determined besides renal analysis of oxidative stress and inflammatory indices.
Results: SAC pretreatment at a dose of 100 mg/kg for 1 week before CCL4 challenge significantly and markedly reduced level of blood urea nitrogen (BUN), malondialdehyde (MDA), reactive oxygen species (ROS), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and significantly enhanced superoxide dismutase (SOD) activity and with no significant effect on creatinine and catalase activity. In addition, such valuable effects were not observed for SAC at a dose of 25 mg/kg in CCL4-exposed group.
Conclusion: Findings of this study indicated beneficial effect of SAC subsequent to CCL4-induced kidney injury that is partly mediated through its regulation of oxidative and inflammatory events and upregulating some of the antioxidants.


  1. Lozano R, Naghavi M, Foreman K, Lim S, Shibuya K, Aboyans V, et al. Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet 2012;380(9859):2095-128.
  2. Zhang Q, Qi J, Luo Q, Wu M, Zhang L, Qin L, et al. Yishen Xiezhuo formula ameliorates the development of cisplatin-induced acute kidney injury by attenuating renal tubular epithelial cell senescence. Annals of Translational Medicine 2022;10(24):1392.
  3. Kalantar-Zadeh K, Jafar TH, Nitsch D, Neuen BL, Perkovic V. Chronic kidney disease. Lancet 2021;398(10302):786-802.
  4. Wei Y-y, Zhang Y-n, Wang H, Ga Y, Fan Y, Wang Q, et al. Mori fructus aqueous extracts attenuate carbon tetrachloride-induced renal injury via the Nrf2 pathway and intestinal flora. Ecotoxicology and Environmental Safety 2022;245:114118.
  5. Dai C, Xiao X, Li D, Tun S, Wang Y, Velkov T, et al. Chloroquine ameliorates carbon tetrachloride-induced acute liver injury in mice via the concomitant inhibition of inflammation and induction of apoptosis. Cell Death & Disease 2018;9(12):1164.
  6. Colín-González AL, Ali SF, Túnez I, Santamaría A. On the antioxidant, neuroprotective and anti-inflammatory properties of S-allyl cysteine: An update. Neurochemistry International 2015;89:83-91.
  7. Shao Z, Pan Z, Lin J, Zhao Q, Wang Y, Ni L, et al. S-allyl cysteine reduces osteoarthritis pathology in the tert-butyl hydroperoxide-treated chondrocytes and the destabilization of the medial meniscus model mice via the Nrf2 signaling pathway. Aging (Albany NY) 2020;12(19):19254-72.
  8. Wang Y, Wang HL, Xing GD, Qian Y, Zhong JF, Chen KL. S-allyl cysteine ameliorates heat stress-induced oxidative stress by activating Nrf2/HO-1 signaling pathway in BMECs. Toxicology and Applied Pharmacology 2021;416:115469.
  9. Khajevand-Khazaei MR, Azimi S, Sedighnejad L, Salari S, Ghorbanpour A, Baluchnejadmojarad T, et al. S-allyl cysteine protects against lipopolysaccharide-induced acute kidney injury in the C57BL/6 mouse strain: Involvement of oxidative stress and inflammation. International Immunopharmacology 2019;69:19-26.
  10. Chiu YJ, Chou SC, Chiu CS, Kao CP, Wu KC, Chen CJ, et al. Hepatoprotective effect of the ethanol extract of Polygonum orientale on carbon tetrachloride-induced acute liver injury in mice. Journal of Food and Drug Analysis 2018;26(1):369-79.
  11. Rousta AM, Mirahmadi SM, Shahmohammadi A, Ramzi S, Baluchnejadmojarad T, Roghani M. S-allyl cysteine, an active ingredient of garlic, attenuates acute liver dysfunction induced by lipopolysaccharide/ d-galactosamine in mouse: Underlying mechanisms. Journal of Biochemical and Molecular Toxicology 2020:e22518.
  12. Arya A, Sethy NK, Singh SK, Das M, Bhargava K. Cerium oxide nanoparticles protect rodent lungs from hypobaric hypoxia-induced oxidative stress and inflammation. International Journal of Nanomedicine 2013;8:4507.
  13. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 1976;72(1-2):248-54.
  14. Platt E, Klootwijk E, Salama A, Davidson B, Robertson F. Literature review of the mechanisms of acute kidney injury secondary to acute liver injury. World Journal of Nephrology 2022;11(1):13-29.
  15. Sakayanathan P, Loganathan C, Thayumanavan P. Protection of pancreatic beta cells against high glucose-induced toxicity by astaxanthin-s-allyl cysteine diester: alteration of oxidative stress and apoptotic-related protein expression. Archives of Physiology and Biochemistry 2022:1-9.
  16. Jiang L, Li Y, Wang F, Zhang X, Zhao R. Protective Effect of S-Allyl Cysteine Against Neonatal Asthmatic Rats. Dose Response 2020;18(4):1559325820982189.