Journal of Basic and Clinical Pathophysiology

Journal of Basic and Clinical Pathophysiology

Antifungal effect of Heracleum persicum on standard Candida dublinensis strain: An in Vitro study

Document Type : Research Paper

Authors
Fatemeh Azizi 1
Sayed Shojaedin Shayehg 2
Mohhamad Amin Bafandeh 2
Alireza Naeini 3
1 Faculty of Dentistry, Shahed University, Tehran, Iran
2 Department of Prosthodontics, Faculty of Dentistry, Shahed University, Tehran, Iran
3 Department of Parasitology and Mycology, Faculty of Medicine, Shahed University, Tehran, Iran
10.22070/jbcp.2024.19038.1178
Abstract
Background and Objective: The aim of this study was to investigate the antifungal effects of aqueous, alcoholic, acetone extracts, and essential oil of Heracleum persicum seeds (Golpar) against standard Candida dublinensis (C.d) strain.
Materials and Methods: The antifungal effects were evaluated using the agar well diffusion method. Additionally, the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) were determined using the serial dilution method. Standard strain C. d was investigated, and Nystatin was used as the positive control.
Results: The mean diameter of growth inhibition zones for the alcoholic and acetone extracts and essential oil of Golpar seeds were reported in millimeters as follows: 13 mm and 10 mm and 15 mm for C.d, respectively. Based on the results, the MIC and MFC of the alcoholic extract of Golpar seeds against C.d were reported as 40 mg/ml and 40 mg/ml. Additionally, the MIC and MFC of the acetone extract of Golpar seeds against C. d were reported as 14 mg/ml and 28 mg/ml, respectively. Moreover, MIC of the essential oil of Golpar seeds against C.d was 0.5 mg/ml and the MFC was 1 mg/ml.
Conclusion: According to the results, the essential oil and the alcoholic and acetone extracts of Golpar seeds demonstrated promising antifungal and anti- Candidal activity against C.d. Therefore, based on the findings of this study and further research on the extracts and essential oil of Golpar, their antifungal and anti-Candidal effects could be utilized in the formulation of mouthwashes for patients with removable dentures suffering from Oral Candidiasis.
Keywords
Heracleum persicum
Golpar
C. dublinensis
Mouthwash
Removable Dentures
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Journal of Basic and Clinical Pathophysiology
Volume 12, Issue 2
October 2024
Pages 17-21