Journal of Basic and Clinical Pathophysiology

Journal of Basic and Clinical Pathophysiology

Effect of chronic L-arginine consumption on albumin level and epidermal growth factor receptor expression in the ovaries of aged female Wistar rats

Document Type : Research Paper

Authors
Mahsa Davarpanah
Manizheh Karami
Fatemeh Lakzaei
Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
10.22070/jbcp.2024.19452.1180
Abstract
Background and Objective: Sex hormone levels decrease in ovarian aging, causing follicular cysts and low levels of the ovarian albumin/epidermal growth factor receptor (EGFR). L-arginine, a nitric oxide (NO) precursor, plays a role in preventing ovarian aging. We intended to show whether chronic L-arginine consumption has anti-aging benefits on the ovary.
Materials and Methods: Wistar rats (250 g, 36 weeks) were divided into two groups (control and experimental). The control group received 1 mL/kg saline intraperitoneally (i.p.). The experimental group received L-arginine (5, 25 and 50 mg/kg) and L-NAME (5 and 25 mg/kg) alone or together for 3, 5, 9 and 21 days, once a day. At the end, blood samples were taken from rats under deep anesthesia, and serum albumin levels were measured using an ELISA kit. The ovaries were then surgically removed and subjected to biometric, histopathological (Hematoxylin-Eosin and Evans blue) and immunohistopathological tests for EGFR. All data were analyzed using analysis of variance (ANOVA) at α=0.05.
Results: L-arginine reduced the number of follicular cysts in the shortest period (3 days) at the lowest dose (5 mg/kg) and improved serum and ovarian albumin levels as well as EGFR levels in ovaries compared to treatment with L-NAME/L-arginine. These phenomena indicate the involvement of NO. High-dose L-arginine (regardless of duration) increased the number of follicular cysts. Weight change was not statistically significant.
Conclusion: L-arginine in short periods and with low concentration can reduce ovarian aging problems by increasing the level of NO.
Keywords
Nitric oxide
L-NAME
Albumin
EGF receptor
Aging
  1. Wang X, Wang L, Xiang W. Mechanisms of ovarian aging in women: a review. J Ovarian Res 2023; 16: 67.
  2. Isola JVV, Ko S, Ocañas SR, Stout MB. Role of Estrogen Receptor α in Aging and Chronic Disease. Adv Geriatr Med Res. 2023; 5(2): e230005.
  3. Gomi I, Fukushima H, Shiraki M, Miwa Y, Ando T, Takai K, et al. Relationship between serum albumin level and aging in community-dwelling self-supported elderly population. J Nutr Sci Vitaminol. 2007; 53: 37-42.
  4. Rongo C. Epidermal growth factor and aging: A signaling molecule reveals a new eye opening function. Aging. 2011; 3(9): 896-905.
  5. Gad MZ. Anti-aging effects of L-arginine. J Adv Res. 2010; 1(3); 169-177.
  6. Mirzaei F, Khazaei M. The role of nitric oxide in biological systems of the body: a systematic review. J Mazand Univ Med Sci. 2016; 27(150): 192-222.
  7. Houshmand M. Dorland's Book of Medical Culture, by Dorland William Alexander Newman. Farhang Maaser Publications, Tehran, 1385.
  8. Rytlewski K, Olszanecki R, Korbut Z, Zdebski Z. Effects of prolonged oral supplementation with l-arginine on blood pressure and nitric oxide synthesis in preeclampsia. European Journal of Clinical Investigation, 2005; 35(Issue 1): 32-37.
  9. Donato AJ, Machin DR, Lesniewski LA. Mechanisms of dysfunction in the aging vasculature and role in age-related disease. Circ Res. 2018; 123, 825-848.
  10. Ahmad P, Latef AAA, Hashem A, Abd-Allah EF, Gucel S, Tran LSP. Nitric oxide mitigates salt stress by regulating levels of osmolytes and antioxidant enzymes in chickpea, Front Plant Sci. 2016; 7: 1-10.
  11. Movahedin M, Karoji SM, Anjom-Rooz AH. Evaluation of the effects of epidermal growth factor on the development of mouse embryos in the pre-implantation stages. Iran J Anat Sci. 2012; 1(2): 3.
  12. Goli P, Yazdi M, Heidari-Beni M, Kelishadi R. Growth Hormone Response to L-Arginine Alone and Combined with Different Doses of Growth Hormone-Releasing Hormone: A Systematic Review and Meta-Analysis. Int J Endocrinol. 2022; 2022: e8739289. 10.1155/2022/8739289.
  13. 13. Oda K, Matsuoka Y, Funahashi A, Kitano H. A comprehensive pathway map of epidermal growth factor receptor signaling. Mol Syst Biol. 2005; 1. doi:10.1038/msb4100014.