Antiepileptic effect of nobiletin in PTZ-induced catamenial seizures in the rat

Document Type : Research Paper

Authors

1 Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

Background and Objective: Catamenial epilepsy is a seizure pattern associated with the menstrual cycle that happens in specific women with epilepsy while menstruating. Nobiletin is a type of polymethoxy flavonoid and with many beneficial physiological effects. This study aimed to determine antiepileptic effect of nobiletin in PTZ-induced catamenial seizures in rats.
Materials and Methods: Thirty female Wistar rats, separated into 5 experimental groups based on their estrus cycle stage (proestrus, estrus, metestrus, diestrus). In the control group, rats received saline, while in experiment 2, the animals were given VPA (75 mg/kg). During experiments 3-5, animals received nobiletin at doses 12.5, 25, and 50 mg/kg, respectively. Then, the animals received an 80 mg per kilogram intraperitoneal injection of PTZ (80 mg/kg). Following the start of the seizure, the animals' actions were observed for a period of 30 minutes to evaluate the onset of ITMS and ITTS. Experiments were conducted between 9 a.m. and 12 p.m. to minimize the influence of circadian rhythm on vulnerability to seizures.
Results: Based on findings, nobiletin (20 and 30 mg/kg) increased the initiation time myoclonic seizures (ITMS) and initiation time of tonic-clonic seizures (ITTS) and decreased seizure duration (SD) after PTZ administration in rats during different stages of the estrous cycle (p<0.05). The effects of nobiletin were more pronounced during metestrus and diestrus in comparison to proestrus and estrus (p<0.05).
Conclusion: Findings suggested that nobiletin has antiepileptic effect in PTZ-induced catamenial seizure in rat.

Keywords

References

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Journal of Basic and Clinical Pathophysiology
Volume 13, Issue 1 - Serial Number 1
February 2025
Pages 44-49

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