Preconditioning Effect of Aerobic Exercise with Vitamin D3 Intake on VEGF Levels in 6-OHDA-Lesioned Rat Model of Parkinson's Disease

Document Type : Research Paper


1 Department of Exercise Physiology, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran

2 Islamic Azad University Sari, Iran, Sari

3 Exercise physiology department،Facuality of Islamic Azad university,sari,Iran


Background and Objective: The purpose of this study was to investigate the preconditioning effect of aerobic exercise with vitamin D3 consumption on vascular endothelial growth factor (VEGF) level in the 6-hydroxydopamine (6-OHDA)-lesioned rat model of Parkinson’s disease.
Materials and Methods: Forty-eight male rats weighing 250-300 g were randomly assigned to 6 groups: healthy control, Parkinson’s control, sham, exercise-Parkinson’s, vitamin-Parkinson’s, and vitamin+exercise-Parkinson’s groups. The exercise groups exercised for 4 weeks, 5 days per week for 30 minutes on a treadmill at the speed of 15 m/min and with the slope of zero. The vitamin groups received vitamin D3 for 4 weeks, 2 days per week and at a dose of 1 mg/kg of body weight. After 4 weeks of exercising and taking the vitamin, an experimental model of Parkinson's disease was developed using stereotactic surgery and injection of 6-OHDA into the striatum. Three weeks later, apomorphine-induced rotational test was conducted in order to verify the parkinsonian condition in rats and then VEGF levels in the striatum were measured by ELISA method after the isolation and extraction of the striatum of the rats’ brain.
Results: The results showed that four weeks of treadmill aerobic exercise in combination with vitamin D3 intake before 6-hydroxydopamine injection could significantly increase VEGF levels in the striatum (pConclusion: The results of this study demonstrated that preconditioning with aerobic exercise combined with D3 intake can increase the protection of dopaminergic neurons against 6-hydroxydopamine-induced damage by increasing the levels of VEGF and thus play a protective role against Parkinson's disease.


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