Journal of Basic and Clinical Pathophysiology

Journal of Basic and Clinical Pathophysiology

Antimicrobial Effects of Zinc Oxide and Silver Nitrate Nanoparticles on S. aureus, A. baumannii and P. aeruginosa

Document Type : Research Paper

Authors
Mohammad Niakan 1
Iman Pouladi 1
Rasoul Kaviani 1
Elham Esmaili 2
1 Department of Medical Microbiology, Faculty of Medicine, Shahed University of Medical Sciences, Tehran, Iran.
2 School of Medicine, Shahed University, Tehran, Iran
10.22070/jbcp.2019.3672.1105
Abstract
Background and Objective: Nanoparticles have been introduced as novel antimicrobial agents because of their properties that are different from their bulk properties. Present study was aimed to investigate antimicrobial activity of silver nitrate and zinc oxide nanoparticles against three main bacteria responsible for nosocomial infections, S. aureus, P. aeruginosa and A. baumannii.
Materials and Methods: Solutions of nanoparticles were prepared at various concentrations (31.5-4000 ppm) in a serial method. Disks with various concentrations of nanoparticles were then placed on bacterial cultures for 24 hours and diameter of inhibition was measured after 24 hours of exposure to nanoparticle in incubator. Using a diagram without statistical analysis, diameters of inhibition were compared between various concentrations and kinds of bacteria. Analysis of variance was used to compare the diameter of inhibition between bacteria based on a variety of nanoparticles regarding their concentration.
Results: Nanoparticles of zinc oxide made an inhibitory diameter of 13.6 mm at highest concentration to 7 mm at lowest concentration of nanoparticle for S. aureus. For this bacterium, silver nitrate nanoparticle had a larger inhibitory diameter (16.33 mm to 8.67 mm). Zinc oxide nanoparticle did not have an inhibitory effect on P. aeruginosa and A. baumannii. The maximum inhibitory diameter of silver nitrate nanoparticle on P. aeruginosa and A. baumannii was measured 13.33 mm and 22.67 mm for P. aeruginosa and A. baumannii, respectively. For both bacteria, inhibitory area reached to zero at a concentration of 125 ppm. Inhibitory areas of silver nitrate were significantly greater than those for zinc oxide (p<0.001).
Conclusion: In summary, silver nitrate nanoparticles have greater antimicrobial activity. Antimicrobial activity of zinc oxide nanoparticles was restricted to gram-positive bacteria.
Keywords
Antimicrobial activity
Nanoparticles
Silver nitrate
Zinc oxide
S. aureus
P. aeruginosa
A. baumannii
  1. Hajipour MJ, Fromm KM, Akbar Ashkarran A, Aberasturi D, Larramendi IR, Rojo T, et al. Antibacterial properties of nanoparticles. Trends in Biotechnology 2012; 30(10): 499-511.
  2. Hernandez-Sierra JF, Ruiz F, Cruz Pena DC, Martinez-Gutierrez F, Martinez AE, Pozos Guillen A, et al. The antimicrobial sensitivity of Streptococcus mutans to nanoparticles of silver, zinc oxide, and gold. Nanomedicine: Nanotechnology Biology and Medicine 2008; 4(3): 237-240.
  3. Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for gram -negative bacteria. Journal of Colloid and Interface Science 2004; 275(1): 177-82.
  4. Kim SH, Lee H, Ryu DS, Choi SJ, Lee DS. Antibacterial Activity of Silver-nanoparticles Against Staphylococcus aureus and Escherichia coli. Journal of Microbiology and Biotechnology 2011; 39(1): 77-85.
  5. Dunn PM , Carl C. The prevention of ophthalmia neonatorum. Archives of Disease in Childhood-Fetal and Neonatal Edition 2000; 83: 158-159.
  6. Banoee M, Seif S, Nazari ZE, Jafari P, Shahverdi HR, Moballegh A, et al. ZnO nanoparticles enhanced antibacterial activity of ciprofloxacin against Staphylococcus aureus and Escherichia coli. Journal of Biomedical Materials Research 2010; 93: 557-561.
  7. Medina C, Santos M, Radomski A, Corrigan O, Radomski M. Nanoparticles: Pharmacological and toxicological significance. Journal of Pharmacology 2007; 150: 552-558.
  8. Samarghandi MR, Hoseinzadeh E, Alikhani MY. Susceptibility constant and death kinetic of Escherichia coli and Staphylococcus aureus to zinc oxide and copper oxide nanoparticles. Hamadan University of Medical Sciences and Health Services 2012.(MSc thesis) [ Persian]
  9. Hoseinzadeh E, Samarghandi MR, Alikhani MY, Asgari Gh, Roshanaei Gh. Effect of zinc oxide (ZnO) nanoparticles on death kinetic of gram negative and positive bacterium. Journal of Babol University of Medical Sciences 2012; 14(5): 13-19. [ Persian]
  10. Alizadeh M. Antibacterial effects of nanosilver on some hospital infections. Tabriz University of Medical Sciences 2011. (MSc thesis) [ persian]
  11. Lkhagvajav N, Yasa I, Celik E, Koizhaiganova M, Sari O. Antimicrobial activity of colloidal silver nanoparticles prepared by sol-gel method. Digest Journal of Nanomaterials and Biostructures 2011; 6: 149-154.
  12. Aruna JK, Arunachalam J. Assessment of antibacterial activity of silver nanoparticles on Pseudomonas aeruginosa and its mechanism of action. World Journal of Microbiology and Biotechnology 2010; 1007(10): 569-677.
  13. Amirulhusni A, Palanisamy NK, Mohd-Zain Z, Jian Ping L, Durairaj R. Antibacterial effect of silver nanoparticles on multi drug resistant Pseudomonas aeruginosa. World Academy of Science Engineering and Technology 2012; 67: 258-261.
  14. Niakan S, Niakan M, Hesaraki S, Nejadmoghaddam MR, Moradi M, Hanafiabdar M, et al . Comparison of the Antibacterial Effects of Nanosilver With 18 Antibiotics on Multidrug Resistance Clinical Isolates of Acinetobacter baumannii. Jundishapur Journal of Microbiology 2013 ; 6(5): e8341.
  15. Sintubin L, De Gussem B, Vandermeeren P, Pycke B, Verstracte W, Boon N. The antibacterial activity of biogenic silver and its mode of action. Journal of Microbiology and Biotechnology 2011; 31: 113-189.
  16. Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of antimicrobials. Biotechnology Advances 2009; 27(1): 76-83.
    17. Raghupathi KR, Koodali RT, Manna AC. Size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticles. Langmuir 2011; 27(7): 4020-4028.
  17. Sotiriou GA, Paratsinis SE. Antibacterial activity of nanosilver ions and particles. Journal of Environment and Scientific Technology 2010; 15: 5649-54.
  18. Choi O, Deng KK, Kim NJ, Rossjr L, Surampalli RY, Hu Z. The inhibitory effects of silver nanoparticles, silver ions, and silver chloride colloids on microbial growth. Water Research 2008; 42(12): 3066-3074. 

Journal of Basic and Clinical Pathophysiology
Volume 7, Issue 1
March 2019
Pages 27-30