Isolation and determination of antibiotic resistance patterns of urinary tract infections pathogens in patients who referred to Pol-e Dokhtar medical centers during 2016-2018

Document Type: Research Paper

Authors

1 Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran

2 Department of Biology, Faculity of science, Shahid Chamran University of Ahvaz, Iran

3 Department of Microbiology, Kerman Branch, Islamic Azad university, Kerman, Iran

10.22070/jbcp.2020.4849.1122

Abstract

Background: Urinary tract infection (UTI) is one of the most common human bacterial infections. The frequent use of antibiotics is associated with increased resistance of many bacterial agents to the treatment.
Materials and Methods: A total of 9456 urine specimens were collected from patients referring to Pol-e Dokhtar health centers. All urine samples were inoculated on blood agar plates (BAP) and eosin methylene blue (EMB) agar. Those specimens positive for selected pathogen were finally identified. The antimicrobial susceptibility and resistance patterns of the UTI pathogens to common antibiotics were determined using disk diffusion method according to Clinical and Laboratory Standards Institute (CLSI) guidelines.
Results: Out of 9456 investigated urine samples, 1036 samples (10.95%) were positive, of which 823 samples (79.4%) belonged to female subjects and 213 samples (20.6%) belonged to males. The most common identified urine tract pathogens were Escherichia coli (69.4%), Staphylococcus epidermidis (15.15%) and Staphylococcus aureus (7.05%). Also, co-trimoxazole (sulfamethoxazole/trimethoprim) displayed the highest antibiotic resistance (58.1%) and nitrofurantoin (90.1%) and gentamicin (88%) were shown to be the most frequent cause of sensitivity.
Conclusion: The findings of our study demonstrate that E. coli is the most common UTI pathogen. Given the high antibiotic resistance of co-trimoxazole, it is recommended to avoid indiscriminate consumption of this antibiotic and perform antibiogram tests for the treatment of UTIs.

Keywords


  1. Madaen SK, Stakhri R, Ghaniafshord H, Fazl-Farhadi V. The Prevalence and Type of Urinary Tract Infection in Patients with Urethral Catheter in Urology Department of Imam Reza Hospital. Journal of Tabriz University of Medical Sciences 2016; 38(4):72-75.
  2. Baghani Aval HR, Ekrami Toroghi M, Haghighi F, Tabarraie Y. Common Bacterial Factors of Urinary Tract Infections and Determining their Antibiotic Resistance in Hospitalized and out Patients Referred to the Vase’ee Hospital in Sabzevar in 2016. Journal of Sabzevar University of Medical Sciences 2018; 25 (5): 687-693
  3. Ahanjan M, Haghshenas MR, Naghshvar F, Bairamvand E. Prevalance of Urinary Tract Infection in Patients Attending Imam Khomeini Hospital, Sari, 2010-2011. Journal of Mazandaran University of Medical Sciences 2013; 23(Supple 1): 82-86 (Persian).
  4. Ramirez-Castillo FY, Moreno-Flores AC, Avelar-Gonzalez FJ, Maraquez-Diaz F, Harel J, Guerrero-Barrera AL. An evaluation of multidrug-resistant Escherichia coli isolates in urinary tract infections from Aguascalientes, Mexico: cross- sectional study. Annals of Clinical Microbiology and Antimicrobials 2018;17(1):34.
  5. Mohammadi S, Ramazanzadeh R, Zandi S, Rouhi S, Mohammadi B. Isolation and antibiotic resistance pattern determination of bacteria causing urinary tract infections in patients referred to Sanandaj`s Tohid Hospital, 2013-2014. Journal of Kurdistan University of Medical Sciences 2015;16(50):55-62.
  6. Jarsiah P, Alizadeh A, Mehdizadeh E, Ataee R, Khanalipour N. Evaluation of Antibiotic Resistance Model of Escherichia Coli in Urine Culture Samples at Kian Hospital Lab in Tehran, 2011-2012. Journal of Mazandaran University of Medical Sciences 2014; 24: 78-83 (Persian).
  7. Rasoulzadeh Bidgoli M, Golmakani N, Ebrahimzadeh Zagmi S, Nasiri S. Cranberry Effects on Prevention of Urinary Tract Infection. Journal of Student Research Committee Sabzevar University of Medical SciŲ§ence. 2017;22(41): 16-22 .
  8. Barari Sawadkouhi R, Sorkhi H, Pournasrollah M, Bijani A, Babazadeh N, Baleghi Damavand S. Antibiotic Resistance of Bacteria Causing Urinary Tract Infections in Children Hospitalized in Amirkola Children Hospital during 2010-2011. Journal of Babol University of Medical Sciences 2013; 15(5): 89-94 .
  9. Heidari-Soureshjani E, Heidari M, Doosti A. Epidemiology of urinary tract infection and antibiotic resistance pattern of Escherichia coli in patients referred to Imam Ali hospital in Farokhshahr, Chaharmahal va Bakhtiari, Iran. Journal of Shahrekord University of Medical Sciences 2013; 15(2): 9-15 .
  10. Mahmoudi H, Emadmomtaz H, Karimitabar Z, Emam AH, Khanali S, Alikhani MY. Prevalence of asymptomatic urinary tract infection in primary school children of Hamadan City and drug resistance of isolated microorganisms in 2014. Pajouhan Scientific Journal 2015;13(3): 8- 14 .
  11. Mohammadi S,Argha K, Akhzarifar N, Panahi Z, Pakzad I, Sayehmiri K. Analysis of Antibiotic Resistance in Bacterial Strains Isolated from Urine Cultures of Patients Referred to Clinical Laboratories of Ilam City during 2012. Journal of Ilam University of Medical Sciences 2016.;24(2) .
  12. Etebarzadeh Z, Oshaghi M, Mozafari NA. Evaluation of relationship between phylogenetic typing and antibiotic resistance of uropathogenic Escherichia coli. Journal of Microbial World 2012;3(3) .
  13. Raeeszadeh M, Ahmadi E, Shafiee M. Identification of the antibiotic resistance patterns in bacteria isolated from urinary tract infections in patients admitted to Shahid Ghazi Hospital Sanandaj in the first 6 months of 2014. Razi Journal of Medical Sciences 2016;23(147) .
  14. Hejazi F, Ahanjan M, Akha O, Salehiyan M. Phenotypic Study of Urinary Tract Infection Producing Bacteria and Antibiotic Resistance Pattern in Diabetic Patients. Journal of Mazandaran University of Medical Sciences 2018; 28 (163): 38-46 (Persian)
  15. Abedi Samakoosh M, Aghaei N, Babamahmodi F,Dawodi AR. Frequency and Pattern of Urinary Pathogens and Their Antibiotic Resistance in Patients with Urinary Tract Infection. Journal of Mazandaran University of Medical Sciences 2015; 25(131): 155-158 (Persian).
  16. Mojabi SM, Ranjbar R. The Frequency of Escherichia coli Strain Isolates Causing Urinary Tract Infections in the presence of Pathogenic Aerobactin Genes. Journal of Babol University of Medical Sciences 2015;17(5):31-6.
  17. Klingeberg A, Noll I, Willrich N, Feig M, Emrich D, Zill E, et al. Antibiotic-Resistant Escherichia coli in Uncomplicated Community-Acquired Urinary Tract Infection. Deutsches Ärzteblatt International 2018;115(29-30): 494- 500 .
  18. Asafo-Adjei K, Mensah JE, Labi AK, Dayie NTKD, Donkor ES. Urinary Tract Infections among Bladder Outlet obstruction patients in Accra, Ghana: Aetiology, Antibiotic Resistance, and Risk Factors. Diseases 2018;6(3):65.
  19. Erdem I, Kara Ali R, Ardic E, Elbasan Omar S, Mutlu R, Topkaya AE. Community-acquired Lower Urinary Tract Infections: Etiology, Antimicrobial, and Treatment Results in Femal Patients. Journal of Global Infectious Diseases 2018;10(3): 129- 132 .
  20. Moulana Z, Asgharpour F, Ramezani T. Frequency of the Bacterial Causing Agents in Urinary Tract Infection and Antibiotic Pattern Samples Sent to Razi Laboratory, Babol 2008-2009. Journal of Rafsanjan University of Medical Sciences 2013; 12(6): 489-494. [Farsi]
  21. Guidoni EB, Berezin EN, Nigro S, Santiago NA, Benini V, Toporovski J. Antibiotic resistance patterns of pediatric community-acquired urinary infections. Brazilian Journal of Infectious Diseases 2008;12(4): 321- 3.