Effect of Hydroalcoholic Leaves Extract of Ziziphora tenuior L. on Pain in Male Rats

Document Type: Research Paper

Authors

1 Young Researchers and Elite Club, Kazerun Branch, Islamic Azad University, Kazerun, IRAN.

2 PhD, Departement of basic scienc, Faculty of Veterinary medicine, Kazerun Branch, Islamic Azad University, Kazerun, I.R.Iran.

3 PhD Student in Traditional Medicine, School of Iranian Traditional Medicine, Tehran, Iran.

4 School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

Ziziphora tenuior is one of the traditional medicinal herbs; the main active chemical composition of this herb is an ingredient named as Pulegone which is known well for its anti-inflammatory and analgesic effects. Considering, the side effects of synthetic drugs, researcher’s attention has been attracted to the use of herbal medicines.
In this study, after inducing ear edema via Xylenol, the anti-inflammatory activity of ethanol extracts on vascular permeability in (NMRI) rats was studied. 60 rats were divided into 6 groups of 10 as follows: Control (no treatment), sham (receiving physiological saline), groups 3, 4, and 5 treated with Ziziphora tenuior extracts which received doses of 20, 50 and 100 milligrams per kilogram of body weight, respectively, and groups 6 through 10 which received Dexamethasone at a concentration of 10 mg per kg of body weight in the amount of 0/2 milliliters.
The results of the present study showed that intraperitoneal injection of Ziziphora tenuior extracts at doses of 50 and 100 mg per kg body weight, results in a significant decrease in inflammation induced via Xylenol in mature male rats (p≤ 0.05), as like lessening of inflammation via 10 mg body weight of Dexamethasone. Furthermore, the group receiving 100 milligrams per kilograms of Ziziphora tenuior extracts showed less inflammation compared to the group receiving dexamethasone. However, no significant difference was seen.
Ziziphora tenuior extract dose-dependently decreases inflammation of ear edema test induced via Xylenol significantly. Probably, the presence of Flavonoids found in the herb, causes its anti-inflammatory effects

Highlights

  1. Ghorbani ranjbary A, yaryar M, joybar F, Ghorbani N. The analgesic effects of Ziziphora tenuior in adult male mice. Yafteh 16 (4) :62-69.
  2. Naghibi F, Mosaddegh M, Mohammadi Motamed S, Ghorbani A. Labiatae family in folk medicine in Iran from ethnobotany to phamacology.Iranian Journal of Pharmacetical Research 2; 63-79.
  3. Sonboli A, Mujalili MH, Hadian J, Nejad Ebrahimi S, Yousefaade M. Antibacterial activity and composition of the essential oil of Ziziphora clinopodioidessubsp. Bungeana (Juz) Reach from Iran. Z. Natureforsch 61: 677-680.
    1. Sosa S, Balick MJ, Arvigo R, Esposito RG, Pizza C, Altinier G, et al. Screening of the topical anti-inflammatory activity of some Central American plants. Journal of Ethnopharmacology 2002; 81: 211-15.
    2. Salehi P, Sonboli A, Eftekhar F, Nejad Ebrahimi S, Yousefzadi M. 2005. Essential oil composition,antibacterial and antioxidant activity of the oil and various extracts of Ziziphora clinopodioides subsp. rigida (Boiss.) Reech ffrom Iran. Biological and Pharmaceutical Bulletin 28: 1892-1896.
    3. Konyalioglu S, Qzturk B, Elgin MG. 2006. Comparison of chemical compositions and antioxidant activities of the essential oils of two Ziziphora taxa from Anatolia. Pharm Biol 44: 121-126.
      1. Ozturk S and Ercisli S. Antibacterial activity and chemical constitutions of Ziziphora clinopodioides. Food Control 2007; 18: 535-540.
      2. De Sousa DP, Junior EV, Oliveira FS, De Almeida RN, Nunes XP, Barbosa-Filho JM. 2007. Antinociceptive activity of structural analogues of rotundifolone: structure-activity relationship. Zeitschrift für Natureforschung 62 (1-2): 39-42.

 

  1. Gozde E, Yavasoglu N, Ulku K, Ozturk B. 2006. Antimicrobial activity of endemic Ziziphorataurica subsp. cleonioides (Boiss) essential oil. Acta Pharmaceutica Sciencia 48:55-62.
  2. Nabiuni M, Doostikhah S, Panahandeh S R, Karimzadeh L. 2015. Hydro-alcoholic extract of Ziziphora tenuior L. on polycystic ovary syndrome in Wistar rats. Tehran University Medical Journal 73 (5) :324-333.
  3. Amanlou M, Dadkhah F, Salehnia A, Farsam H. 2005. An anti-inflammatory and anti-nociceptive effects of hydroalcoholic extract of Saturejakhuzistanica Jamzad extract. The Journal of Pharmacy and Pharmaceutical Sciences 8:102-106.
  4. Hossinzadeh H, Ramezani M, and Salmani GA. 2000 Antinociceptive, anti-intoxicity effects of Zataria multiflora Boiss extrcts in mice and rats. Journal of Ethnopharmacology 73:379-85.
  5. Roy-Chaudhury P, Sukhatme VP, Cheung AK. 2006. Hemodialysis vascular access dysfunction: a cellular and molecular viewpoint. Journal of the American Society of Nephrology 17: 1112–1127.
  6. Markiewski MM, Lambris JD. 2007. The Role of Complement in Inflammatory Diseases From Behind the Scenes into the Spotlight. The American Journal of Pathology 171(3):715-727.
  7. Vono M, Taccone M, Caccin P, Gallotta M, Donvito G, Falzoni S, Palmieri E, Pallaoro M, Rappuoli R, Di Virgilio F, De Gregorio E. The adjuvant MF59 induces ATP release from muscle that potentiates response to vaccination. Proceedings of the National Academy of Sciences 2013 24;110(52):21095-100.
  8. Jennette JC, Falk RJ, Hu P, Xiao H. . 2013. Pathogenesis of antineutrophil cytoplasmic autoantibody-associated small-vessel vasculitis. Annual Review of Pathology: Mechanisms of Disease 24;8:139-60.
  9. Mandhane SN, Shah JH, Thennati R. 2011. Allergic rhinitis: an update on disease, present treatments and future prospects. International Immunopharmacology 30;11(11):1646-62.
  10. Joshi B, Sah GP, Basnet BB, Bhatt MR, Sharma D, Subedi K, Pandey J, Malla R. . 2011. Phytochemical extraction and antimicrobial properties of different medicinal plants: Ocimum sanctum (Tulsi), Eugenia caryophyllata (Clove), Achyranthes bidentata (Datiwan) and Azadirachta indica (Neem). Journal of Microbiology and Antimicrobials 30;3(1):1-7.
  11. Kim HP, Son KH, Chang HW, Kang SS. 2004. Anti-inflammatory plant flavonoids and cellular action mechanisms. Journal of Pharmacological Sciences 96(3):229-45.
  12. Pan MH, Lai CS, Ho CT. 2010. Anti-inflammatory activity of natural dietary flavonoids. Food & Function 1(1):15-31.
  13. Kimura Y, Okuda H, and Arichi S. 1985. Studies on Scutellariae radix, Effects of various flavonoids on arachidonate metabolism in leukocytes. Planta Medica 2: 132-6.

 

 

 

 

 

 

 

 

 

  1. Ghorbani ranjbary A, yaryar M, joybar F, Ghorbani N. The analgesic effects of Ziziphora tenuior in adult male mice. Yafteh 16 (4) :62-69.
  2. Naghibi F, Mosaddegh M, Mohammadi Motamed S, Ghorbani A. Labiatae family in folk medicine in Iran from ethnobotany to phamacology.Iranian Journal of Pharmacetical Research 2; 63-79.
  3. Sonboli A, Mujalili MH, Hadian J, Nejad Ebrahimi S, Yousefaade M. Antibacterial activity and composition of the essential oil of Ziziphora clinopodioidessubsp. Bungeana (Juz) Reach from Iran. Z. Natureforsch 61: 677-680.
    1. Sosa S, Balick MJ, Arvigo R, Esposito RG, Pizza C, Altinier G, et al. Screening of the topical anti-inflammatory activity of some Central American plants. Journal of Ethnopharmacology 2002; 81: 211-15.
    2. Salehi P, Sonboli A, Eftekhar F, Nejad Ebrahimi S, Yousefzadi M. 2005. Essential oil composition,antibacterial and antioxidant activity of the oil and various extracts of Ziziphora clinopodioides subsp. rigida (Boiss.) Reech ffrom Iran. Biological and Pharmaceutical Bulletin 28: 1892-1896.
    3. Konyalioglu S, Qzturk B, Elgin MG. 2006. Comparison of chemical compositions and antioxidant activities of the essential oils of two Ziziphora taxa from Anatolia. Pharm Biol 44: 121-126.
      1. Ozturk S and Ercisli S. Antibacterial activity and chemical constitutions of Ziziphora clinopodioides. Food Control 2007; 18: 535-540.
      2. De Sousa DP, Junior EV, Oliveira FS, De Almeida RN, Nunes XP, Barbosa-Filho JM. 2007. Antinociceptive activity of structural analogues of rotundifolone: structure-activity relationship. Zeitschrift für Natureforschung 62 (1-2): 39-42. 
      3. Gozde E, Yavasoglu N, Ulku K, Ozturk B. 2006. Antimicrobial activity of endemic Ziziphorataurica subsp. cleonioides (Boiss) essential oil. Acta Pharmaceutica Sciencia 48:55-62.
      4. Nabiuni M, Doostikhah S, Panahandeh S R, Karimzadeh L. 2015. Hydro-alcoholic extract of Ziziphora tenuior L. on polycystic ovary syndrome in Wistar rats. Tehran University Medical Journal 73 (5) :324-333.
      5. Amanlou M, Dadkhah F, Salehnia A, Farsam H. 2005. An anti-inflammatory and anti-nociceptive effects of hydroalcoholic extract of Saturejakhuzistanica Jamzad extract. The Journal of Pharmacy and Pharmaceutical Sciences 8:102-106.
      6. Hossinzadeh H, Ramezani M, and Salmani GA. 2000 Antinociceptive, anti-intoxicity effects of Zataria multiflora Boiss extrcts in mice and rats. Journal of Ethnopharmacology 73:379-85.
      7. Roy-Chaudhury P, Sukhatme VP, Cheung AK. 2006. Hemodialysis vascular access dysfunction: a cellular and molecular viewpoint. Journal of the American Society of Nephrology 17: 1112–1127.
      8. Markiewski MM, Lambris JD. 2007. The Role of Complement in Inflammatory Diseases From Behind the Scenes into the Spotlight. The American Journal of Pathology 171(3):715-727.
      9. Vono M, Taccone M, Caccin P, Gallotta M, Donvito G, Falzoni S, Palmieri E, Pallaoro M, Rappuoli R, Di Virgilio F, De Gregorio E. The adjuvant MF59 induces ATP release from muscle that potentiates response to vaccination. Proceedings of the National Academy of Sciences 2013 24;110(52):21095-100.
      10. Jennette JC, Falk RJ, Hu P, Xiao H. . 2013. Pathogenesis of antineutrophil cytoplasmic autoantibody-associated small-vessel vasculitis. Annual Review of Pathology: Mechanisms of Disease 24;8:139-60.
      11. Mandhane SN, Shah JH, Thennati R. 2011. Allergic rhinitis: an update on disease, present treatments and future prospects. International Immunopharmacology 30;11(11):1646-62.
      12. Joshi B, Sah GP, Basnet BB, Bhatt MR, Sharma D, Subedi K, Pandey J, Malla R. . 2011. Phytochemical extraction and antimicrobial properties of different medicinal plants: Ocimum sanctum (Tulsi), Eugenia caryophyllata (Clove), Achyranthes bidentata (Datiwan) and Azadirachta indica (Neem). Journal of Microbiology and Antimicrobials 30;3(1):1-7.
      13. Kim HP, Son KH, Chang HW, Kang SS. 2004. Anti-inflammatory plant flavonoids and cellular action mechanisms. Journal of Pharmacological Sciences 96(3):229-45.
      14. Pan MH, Lai CS, Ho CT. 2010. Anti-inflammatory activity of natural dietary flavonoids. Food & Function 1(1):15-31.
      15. Kimura Y, Okuda H, and Arichi S. 1985. Studies on Scutellariae radix, Effects of various flavonoids on arachidonate metabolism in leukocytes. Planta Medica 2: 132-6.

 

 

 

 

 

 

 

 

 

Keywords


  1. Ghorbani ranjbary A, yaryar M, joybar F, Ghorbani N. The analgesic effects of Ziziphora tenuior in adult male mice. Yafteh 16 (4) :62-69.
  2. Naghibi F, Mosaddegh M, Mohammadi Motamed S, Ghorbani A. Labiatae family in folk medicine in Iran from ethnobotany to phamacology.Iranian Journal of Pharmacetical Research 2; 63-79.
  3. Sonboli A, Mujalili MH, Hadian J, Nejad Ebrahimi S, Yousefaade M. Antibacterial activity and composition of the essential oil of Ziziphora clinopodioidessubsp. Bungeana (Juz) Reach from Iran. Z. Natureforsch 61: 677-680.
  4. Sosa S, Balick MJ, Arvigo R, Esposito RG, Pizza C, Altinier G, et al. Screening of the topical anti-inflammatory activity of some Central American plants. Journal of Ethnopharmacology 2002; 81: 211-15.
  5. Salehi P, Sonboli A, Eftekhar F, Nejad Ebrahimi S, Yousefzadi M. 2005. Essential oil composition,antibacterial and antioxidant activity of the oil and various extracts of Ziziphora clinopodioides subsp. rigida (Boiss.) Reech ffrom Iran. Biological and Pharmaceutical Bulletin 28: 1892-1896.
  6. Konyalioglu S, Qzturk B, Elgin MG. 2006. Comparison of chemical compositions and antioxidant activities of the essential oils of two Ziziphora taxa from Anatolia. Pharm Biol 44: 121-126.
  7. Ozturk S and Ercisli S. Antibacterial activity and chemical constitutions of Ziziphora clinopodioides. Food Control 2007; 18: 535-540.
  8. De Sousa DP, Junior EV, Oliveira FS, De Almeida RN, Nunes XP, Barbosa-Filho JM. 2007. Antinociceptive activity of structural analogues of rotundifolone: structure-activity relationship. Zeitschrift für Natureforschung 62 (1-2): 39-42.
  9. Gozde E, Yavasoglu N, Ulku K, Ozturk B. 2006. Antimicrobial activity of endemic Ziziphorataurica subsp. cleonioides (Boiss) essential oil. Acta Pharmaceutica Sciencia 48:55-62.
  10. Nabiuni M, Doostikhah S, Panahandeh S R, Karimzadeh L. 2015. Hydro-alcoholic extract of Ziziphora tenuior L. on polycystic ovary syndrome in Wistar rats. Tehran University Medical Journal 73 (5) :324-333.
  11. Amanlou M, Dadkhah F, Salehnia A, Farsam H. 2005. An anti-inflammatory and anti-nociceptive effects of hydroalcoholic extract of Saturejakhuzistanica Jamzad extract. The Journal of Pharmacy and Pharmaceutical Sciences 8:102-106.
  12. Hossinzadeh H, Ramezani M, and Salmani GA. 2000 Antinociceptive, anti-intoxicity effects of Zataria multiflora Boiss extrcts in mice and rats. Journal of Ethnopharmacology 73:379-85.
  13. Roy-Chaudhury P, Sukhatme VP, Cheung AK. 2006. Hemodialysis vascular access dysfunction: a cellular and molecular viewpoint. Journal of the American Society of Nephrology 17: 1112–1127.
  14. Markiewski MM, Lambris JD. 2007. The Role of Complement in Inflammatory Diseases From Behind the Scenes into the Spotlight. The American Journal of Pathology 171(3):715-727.
  15. Vono M, Taccone M, Caccin P, Gallotta M, Donvito G, Falzoni S, Palmieri E, Pallaoro M, Rappuoli R, Di Virgilio F, De Gregorio E. The adjuvant MF59 induces ATP release from muscle that potentiates response to vaccination. Proceedings of the National Academy of Sciences 2013 24;110(52):21095-100.
  16. Jennette JC, Falk RJ, Hu P, Xiao H. . 2013. Pathogenesis of antineutrophil cytoplasmic autoantibody-associated small-vessel vasculitis. Annual Review of Pathology: Mechanisms of Disease 24;8:139-60.
  17. Mandhane SN, Shah JH, Thennati R. 2011. Allergic rhinitis: an update on disease, present treatments and future prospects. International Immunopharmacology 30;11(11):1646-62.
  18. Joshi B, Sah GP, Basnet BB, Bhatt MR, Sharma D, Subedi K, Pandey J, Malla R. . 2011. Phytochemical extraction and antimicrobial properties of different medicinal plants: Ocimum sanctum (Tulsi), Eugenia caryophyllata (Clove), Achyranthes bidentata (Datiwan) and Azadirachta indica (Neem). Journal of Microbiology and Antimicrobials 30;3(1):1-7.
  19. Kim HP, Son KH, Chang HW, Kang SS. 2004. Anti-inflammatory plant flavonoids and cellular action mechanisms. Journal of Pharmacological Sciences 96(3):229-45.
  20. Pan MH, Lai CS, Ho CT. 2010. Anti-inflammatory activity of natural dietary flavonoids. Food & Function 1(1):15-31.
  21. Kimura Y, Okuda H, and Arichi S. 1985. Studies on Scutellariae radix, Effects of various flavonoids on arachidonate metabolism in leukocytes. Planta Medica 2: 132-6.