Antimicrobial Effects of Zinc Oxide and Silver Nitrate Nanoparticles on S. aureus, A. baumannii and P. aeruginosa

Document Type: Research Paper


1 Department of Medical Microbiology, Faculty of Medicine, Shahed University of Medical Sciences, Tehran, Iran.

2 School of Medicine, Shahed University, Tehran, Iran


Background and Objective: Nanoparticles have been introduced as novel antimicrobial agents because of their properties that are different from their bulk properties. Present study was aimed to investigate antimicrobial activity of silver nitrate and zinc oxide nanoparticles against three main bacteria responsible for nosocomial infections, S. aureus, P. aeruginosa and A. baumannii.
Materials and Methods: Solutions of nanoparticles were prepared at various concentrations (31.5-4000 ppm) in a serial method. Disks with various concentrations of nanoparticles were then placed on bacterial cultures for 24 hours and diameter of inhibition was measured after 24 hours of exposure to nanoparticle in incubator. Using a diagram without statistical analysis, diameters of inhibition were compared between various concentrations and kinds of bacteria. Analysis of variance was used to compare the diameter of inhibition between bacteria based on a variety of nanoparticles regarding their concentration.
Results: Nanoparticles of zinc oxide made an inhibitory diameter of 13.6 mm at highest concentration to 7 mm at lowest concentration of nanoparticle for S. aureus. For this bacterium, silver nitrate nanoparticle had a larger inhibitory diameter (16.33 mm to 8.67 mm). Zinc oxide nanoparticle did not have an inhibitory effect on P. aeruginosa and A. baumannii. The maximum inhibitory diameter of silver nitrate nanoparticle on P. aeruginosa and A. baumannii was measured 13.33 mm and 22.67 mm for P. aeruginosa and A. baumannii, respectively. For both bacteria, inhibitory area reached to zero at a concentration of 125 ppm. Inhibitory areas of silver nitrate were significantly greater than those for zinc oxide (p<0.001).
Conclusion: In summary, silver nitrate nanoparticles have greater antimicrobial activity. Antimicrobial activity of zinc oxide nanoparticles was restricted to gram-positive bacteria.


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